Delayed Excimer Fluorescence of Acridine Orange Bound to DNA
نویسندگان
چکیده
منابع مشابه
Spectral Properties of Acridine Orange Bound to Dna/rna
DNA/RNA, present in almost every cell, has been a hot research topic in a wide range of areas, including biology, physics, chemistry and polymer engineering. Naturally, to image DNA and facilitate DNA/RNA-related questions becomes an interesting question itself. Since 50 years ago people have been discovering and developing families of fluorescence dyes (from Acridine orange to YOYO-1) with uni...
متن کاملFluorescence decay and quantum yield characteristics of acridine orange and proflavine bound to DNA.
Fluorescence properties (quantum yield, decay curve, lifetime and polarization) of acridine orange and proflavine bound to DNA were examined as a function of nucleotide to dye (P/D) ratio. First, mean fluorescence lifetimes were determined by the phase-shift measurements. The lifetime and quantum yield of acridine orange increased in a parallel fashion with increasing P/D ratio. There was no pa...
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LTHOUGH FLUORESCENCE microscopy had its beginnings over half a century ago, it has only recently gained widespread usage as a research tool, and practical applications have yet to be fully exploited. Blood has been one of the tissues most extensively studied by fluorochroming,’ but few reports have been published in English. In addition, the previously reported saline dilution technics for supr...
متن کاملSupravital Blood Studies, Using Acridine Orange Fluorescence
LTHOUGH FLUORESCENCE microscopy had its beginnings over half a century ago, it has only recently gained widespread usage as a research tool, and practical applications have yet to be fully exploited. Blood has been one of the tissues most extensively studied by fluorochroming,’ but few reports have been published in English. In addition, the previously reported saline dilution technics for supr...
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During human spermiogenesis, the elongated spermatids undergo a plasma membrane remodeling step that facilitates formation of the zona pellucida and hyaluronic acid (HA) binding sites. Various biochemical sperm markers indicated that human sperm bound to HA exhibit attributes similar to that of zona pellucida-bound sperm, including minimal DNA fragmentation, normal shape, and low frequency of c...
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ژورنال
عنوان ژورنال: Bulletin of the Chemical Society of Japan
سال: 1979
ISSN: 0009-2673,1348-0634
DOI: 10.1246/bcsj.52.693